Wetland Ecobiology

fa تأثیر پودر چای سبز (Camellia sinensis) بر برخی شاخص‌های خونی ماهی کپور معمولی (Cyprinus carpio) Study the effect of adding the green tea (Camelliasinensis) powder to diet of cyprinuscarpio and its influens on blood factors and some immune system factors for cyprinuscarpio تخصصي Special پژوهشي Research <span dir="RTL" lang="AR-SA" style="line-height: 115%; font-family: "B Mitra"; font-size: 12pt; mso-ansi-font-size: 11.0pt; mso-ascii-font-family: "Times New Romans"; mso-fareast-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Romans"; mso-ansi-language: EN-US; mso-fareast-language: EN-US; mso-bidi-language: AR-SA;">در این بررسی تأثیر عملکرد گیاه چای سبز (<span style="line-height: 115%; font-family: "Times New Roman","serif"; font-size: 11pt; mso-fareast-font-family: "Times New Roman"; mso-ansi-language: EN-US; mso-fareast-language: EN-US; mso-bidi-language: AR-SA; mso-bidi-font-size: 12.0pt; mso-ascii-theme-font: major-bidi; mso-hansi-theme-font: major-bidi; mso-bidi-font-family: "B Mitra";">Camellia<span style="line-height: 115%; font-family: "Times New Romans","serif"; font-size: 11pt; mso-fareast-font-family: "Times New Roman"; mso-ansi-language: EN-US; mso-fareast-language: EN-US; mso-bidi-language: AR-SA; mso-bidi-font-size: 12.0pt; mso-bidi-font-family: "B Mitra";"> <span style="line-height: 115%; font-family: "Times New Roman","serif"; font-size: 11pt; mso-fareast-font-family: "Times New Roman"; mso-ansi-language: EN-US; mso-fareast-language: EN-US; mso-bidi-language: AR-SA; mso-bidi-font-size: 12.0pt; mso-ascii-theme-font: major-bidi; mso-hansi-theme-font: major-bidi; mso-bidi-font-family: "B Mitra";">sinensis<span dir="RTL" lang="AR-SA" style="line-height: 115%; font-family: "B Mitra"; font-size: 12pt; mso-ansi-font-size: 11.0pt; mso-ascii-font-family: "Times New Romans"; mso-fareast-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Romans"; mso-ansi-language: EN-US; mso-fareast-language: EN-US; mso-bidi-language: AR-SA;">)<span lang="AR-SA" style="line-height: 115%; font-family: "Times New Romans","serif"; font-size: 11pt; mso-fareast-font-family: "Times New Roman"; mso-ansi-language: EN-US; mso-fareast-language: EN-US; mso-bidi-language: AR-SA; mso-bidi-font-size: 12.0pt; mso-bidi-font-family: "B Mitra";"> <span dir="RTL" lang="AR-SA" style="line-height: 115%; font-family: "B Mitra"; font-size: 12pt; mso-ansi-font-size: 11.0pt; mso-ascii-font-family: "Times New Romans"; mso-fareast-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Romans"; mso-ansi-language: EN-US; mso-fareast-language: EN-US; mso-bidi-language: AR-SA;">به‌عنوان یک فیتوبیوتیک بر شاخصهای خونی ماهی کپور معمولی (<span style="line-height: 115%; font-family: "Times New Roman","serif"; font-size: 11pt; mso-fareast-font-family: "Times New Roman"; mso-ansi-language: EN-US; mso-fareast-language: EN-US; mso-bidi-language: AR-SA; mso-bidi-font-size: 12.0pt; mso-ascii-theme-font: major-bidi; mso-hansi-theme-font: major-bidi; mso-bidi-font-family: "B Mitra";">Cyprinus carpio<span dir="RTL" lang="AR-SA" style="line-height: 115%; font-family: "B Mitra"; font-size: 12pt; mso-ansi-font-size: 11.0pt; mso-ascii-font-family: "Times New Romans"; mso-fareast-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Romans"; mso-ansi-language: EN-US; mso-fareast-language: EN-US; mso-bidi-language: AR-SA;">) موردبررسی قرار گرفت. تعداد 360 عدد بچه ماهی کپور معمولی با میانگین وزن اولیه 1<span dir="RTL" lang="AR-SA" style="line-height: 115%; font-family: "Cambria","serif"; font-size: 12pt; mso-ansi-font-size: 11.0pt; mso-fareast-font-family: "Times New Roman"; mso-ansi-language: EN-US; mso-fareast-language: EN-US; mso-bidi-language: AR-SA; mso-bidi-font-family: Cambria;">±<span dir="RTL" lang="AR-SA" style="line-height: 115%; font-family: "B Mitra"; font-size: 12pt; mso-ansi-font-size: 11.0pt; mso-ascii-font-family: "Times New Romans"; mso-fareast-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Romans"; mso-ansi-language: EN-US; mso-fareast-language: EN-US; mso-bidi-language: AR-SA;">22 گرم انتخاب و پس از سازگاری با محیط به‌صورت انتخابی در 12 مخزن با 30 قطعه بچه ماهی ذخیره شد. گیاه چای سبز در 3 سطح 5/1 درصد (تیمار 2)، 3 درصد (تیمار 3) و 5 درصد (تیمار 4) و جیره فاقد چای سبز برای تغذیه گروه شاهد (تیمار 1) مورداستفاده قرار گرفت. هر تیمار در 3 تکرار انجام گرفت. ماهیان روزانه به میزان 3 درصد از وزن بدن مورد تغذیه قرار گرفتند. در پایان دوره پرورشی 45 روزه تعداد 15 قطعه ماهی از هر تیمار به‌صورت تصادفی انتخاب و خون‌گیری از محل قطع ساقه دمی انجام گرفت. شاخصهای خونی شامل شمارش تعداد گلبولهای قرمز <span style="line-height: 115%; font-family: "Times New Roman","serif"; font-size: 11pt; mso-fareast-font-family: "Times New Roman"; mso-ansi-language: EN-US; mso-fareast-language: EN-US; mso-bidi-language: AR-SA; mso-bidi-font-size: 12.0pt; mso-ascii-theme-font: major-bidi; mso-hansi-theme-font: major-bidi; mso-bidi-font-family: "B Mitra";">(RBC)<span dir="RTL" lang="AR-SA" style="line-height: 115%; font-family: "B Mitra"; font-size: 12pt; mso-ansi-font-size: 11.0pt; mso-ascii-font-family: "Times New Romans"; mso-fareast-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Romans"; mso-ansi-language: EN-US; mso-fareast-language: EN-US; mso-bidi-language: AR-SA;">، شمارش تعداد گلبولهای سفید (<span style="line-height: 115%; font-family: "Times New Roman","serif"; font-size: 11pt; mso-fareast-font-family: "Times New Roman"; mso-ansi-language: EN-US; mso-fareast-language: EN-US; mso-bidi-language: AR-SA; mso-bidi-font-size: 12.0pt; mso-ascii-theme-font: major-bidi; mso-hansi-theme-font: major-bidi; mso-bidi-font-family: "B Mitra";">WBC<span dir="RTL" lang="AR-SA" style="line-height: 115%; font-family: "B Mitra"; font-size: 12pt; mso-ansi-font-size: 11.0pt; mso-ascii-font-family: "Times New Romans"; mso-fareast-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Romans"; mso-ansi-language: EN-US; mso-fareast-language: EN-US; mso-bidi-language: AR-SA;">)، هماتوکریت (<span style="line-height: 115%; font-family: "Times New Roman","serif"; font-size: 11pt; mso-fareast-font-family: "Times New Roman"; mso-ansi-language: EN-US; mso-fareast-language: EN-US; mso-bidi-language: AR-SA; mso-bidi-font-size: 12.0pt; mso-ascii-theme-font: major-bidi; mso-hansi-theme-font: major-bidi; mso-bidi-font-family: "B Mitra";">PCV<span dir="RTL" lang="AR-SA" style="line-height: 115%; font-family: "B Mitra"; font-size: 12pt; mso-ansi-font-size: 11.0pt; mso-ascii-font-family: "Times New Romans"; mso-fareast-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Romans"; mso-ansi-language: EN-US; mso-fareast-language: EN-US; mso-bidi-language: AR-SA;">) و هموگلوبین (<span style="line-height: 115%; font-family: "Times New Roman","serif"; font-size: 11pt; mso-fareast-font-family: "Times New Roman"; mso-ansi-language: EN-US; mso-fareast-language: EN-US; mso-bidi-language: AR-SA; mso-bidi-font-size: 12.0pt; mso-ascii-theme-font: major-bidi; mso-hansi-theme-font: major-bidi; mso-bidi-font-family: "B Mitra";">Hb<span dir="RTL" lang="AR-SA" style="line-height: 115%; font-family: "B Mitra"; font-size: 12pt; mso-ansi-font-size: 11.0pt; mso-ascii-font-family: "Times New Romans"; mso-fareast-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Romans"; mso-ansi-language: EN-US; mso-fareast-language: EN-US; mso-bidi-language: AR-SA;">) موردبررسی قرار گرفتند و نتایج نشان داد که میزان گلبولهای سفید، هماتوکریت و هموگلوبین اختلاف معنیداری را با سایر تیمارها داشته است (05/0<span style="line-height: 115%; font-family: "Times New Roman","serif"; font-size: 11pt; mso-fareast-font-family: "Times New Roman"; mso-ansi-language: EN-US; mso-fareast-language: EN-US; mso-bidi-language: AR-SA; mso-bidi-font-size: 12.0pt; mso-bidi-font-family: "B Mitra";">P<span style="line-height: 115%; font-family: "Times New Roman","serif"; font-size: 11pt; mso-fareast-font-family: "Times New Roman"; mso-ansi-language: EN-US; mso-fareast-language: EN-US; mso-bidi-language: AR-SA; mso-bidi-font-size: 12.0pt; mso-bidi-font-family: Cambria;"><<span dir="RTL" lang="AR-SA" style="line-height: 115%; font-family: "B Mitra"; font-size: 12pt; mso-ansi-font-size: 11.0pt; mso-ascii-font-family: "Times New Romans"; mso-fareast-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Romans"; mso-ansi-language: EN-US; mso-fareast-language: EN-US; mso-bidi-language: AR-SA;">) و بیشترین میزان آن در تیمار 5/1 درصد به ترتیب به میزان 19/1<span dir="RTL" lang="AR-SA" style="line-height: 115%; font-family: "Cambria","serif"; font-size: 12pt; mso-ansi-font-size: 11.0pt; mso-fareast-font-family: "Times New Roman"; mso-ansi-language: EN-US; mso-fareast-language: EN-US; mso-bidi-language: AR-SA; mso-bidi-font-family: Cambria;">±<span dir="RTL" lang="AR-SA" style="line-height: 115%; font-family: "B Mitra"; font-size: 12pt; mso-ansi-font-size: 11.0pt; mso-ascii-font-family: "Times New Romans"; mso-fareast-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Romans"; mso-ansi-language: EN-US; mso-fareast-language: EN-US; mso-bidi-language: AR-SA;">95/7 (تعداد در میکرو لیتر<span dir="RTL" lang="AR-SA" style="line-height: 115%; font-family: "B Mitra"; font-size: 12pt; mso-ansi-font-size: 11.0pt; mso-ascii-font-family: Calibri; mso-fareast-font-family: "Times New Roman"; mso-hansi-font-family: Calibri; mso-ansi-language: EN-US; mso-fareast-language: EN-US; mso-bidi-language: AR-SA;">×<span dir="RTL" lang="AR-SA" style="line-height: 115%; font-family: "B Mitra"; font-size: 12pt; mso-ansi-font-size: 11.0pt; mso-ascii-font-family: "Times New Romans"; mso-fareast-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Romans"; mso-ansi-language: EN-US; mso-fareast-language: EN-US; mso-bidi-language: AR-SA;"> 103)، 87/4<span dir="RTL" lang="AR-SA" style="line-height: 115%; font-family: "Cambria","serif"; font-size: 12pt; mso-ansi-font-size: 11.0pt; mso-fareast-font-family: "Times New Roman"; mso-ansi-language: EN-US; mso-fareast-language: EN-US; mso-bidi-language: AR-SA; mso-bidi-font-family: Cambria;">±<span dir="RTL" lang="AR-SA" style="line-height: 115%; font-family: "B Mitra"; font-size: 12pt; mso-ansi-font-size: 11.0pt; mso-ascii-font-family: "Times New Romans"; mso-fareast-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Romans"; mso-ansi-language: EN-US; mso-fareast-language: EN-US; mso-bidi-language: AR-SA;">73/43 (درصد) و 74/0<span dir="RTL" lang="AR-SA" style="line-height: 115%; font-family: "Cambria","serif"; font-size: 12pt; mso-ansi-font-size: 11.0pt; mso-fareast-font-family: "Times New Roman"; mso-ansi-language: EN-US; mso-fareast-language: EN-US; mso-bidi-language: AR-SA; mso-bidi-font-family: Cambria;">±<span dir="RTL" lang="AR-SA" style="line-height: 115%; font-family: "B Mitra"; font-size: 12pt; mso-ansi-font-size: 11.0pt; mso-ascii-font-family: "Times New Romans"; mso-fareast-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Romans"; mso-ansi-language: EN-US; mso-fareast-language: EN-US; mso-bidi-language: AR-SA;">74/6<span lang="AR-SA" style="line-height: 115%; font-family: "Times New Roman","serif"; font-size: 11pt; mso-fareast-font-family: "Times New Roman"; mso-ansi-language: EN-US; mso-fareast-language: EN-US; mso-bidi-language: AR-SA; mso-bidi-font-size: 12.0pt; mso-ascii-theme-font: major-bidi; mso-hansi-theme-font: major-bidi; mso-bidi-font-family: "B Mitra";"> <span dir="RTL" lang="AR-SA" style="line-height: 115%; font-family: "B Mitra"; font-size: 12pt; mso-ansi-font-size: 11.0pt; mso-ascii-font-family: "Times New Romans"; mso-fareast-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Romans"; mso-ansi-language: EN-US; mso-fareast-language: EN-US; mso-bidi-language: AR-SA;">(گرم بر دسی لیتر) بوده است، ولی تعداد گلبولهای قرمز تحت تأثیر هیچ‌کدام از موارد آزمایشی قرار نگرفت (05/0<span style="line-height: 115%; font-family: "Times New Roman","serif"; font-size: 11pt; mso-fareast-font-family: "Times New Roman"; mso-ansi-language: EN-US; mso-fareast-language: EN-US; mso-bidi-language: AR-SA; mso-bidi-font-size: 12.0pt; mso-bidi-font-family: "B Mitra";">P><span dir="RTL" lang="AR-SA" style="line-height: 115%; font-family: "B Mitra"; font-size: 12pt; mso-ansi-font-size: 11.0pt; mso-ascii-font-family: "Times New Romans"; mso-fareast-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Romans"; mso-ansi-language: EN-US; mso-fareast-language: EN-US; mso-bidi-language: AR-SA;">). به‌طورکلی تجویز پودر چای سبز در سطح 5/1 درصد به‌صورت خوراکی باعث تحریک برخی شاخصهای خونی گردید و این ماده به‌عنوان یک محرک در بچه ماهی کپور معمولی قابل‌استفاده است. In this investigation the effects of Camellia sinensis as a phytobiotic on the blood factors of the Cyprinuscarpio was examined. For this purpose 360 juvenile Cyprinuscarpio with the average weight of 22±1g were selected and after being adapted to the environment, the fish were stored in 12 tanks (30 fish in each tank). The Camellia sinensis was added to the diet in three levels 1.5% (treatment 2) 3% (treatment 3) and 5% (treatment 4) and the diet without Camellia sinensis was used for the control group (treatment 1). Each treatment repeated 3 times a day. The fish were fed 3% of their weight. At the end of the 45 day treatment period, 15 fish were randomly selected from each treatment and their blood sample was taken from the caudal vein. The hematology factors including differential red blood cells, white blood cells, hematocrit and hemoglobulin had significant difference white compare to other treatments (P<0/05), and maximum amount of them in 1.5% treatment was equal to 7.95±1.19, 43.73±4.78 and 6.74±0.74, respectively. However, amount of white blood cells was not <a name="OLE_LINK149"></a><a name="OLE_LINK148">affected</a> by experimental factors (P>0.05) and minimum of it (1.68±0.21) was related to 5% green tea treatment. In general, that prescribing Camellia sinensis at 1.5% level orally stimulates some non-specific blood and immune factors and it can be used as the immunity stimulant for the juvenile Cyprinuscarpio. چای سبز, Camellia sinensis, کپور معمولی, Cyprinus carpio, فاکتور خونی, هماتولوژی. Camellia sinensis, Cyprinuscarpio, Blood factor, Hemathology 39 50 http://jweb.ahvaz.iau.ir/browse.php?a_code=A-10-356-4&slc_lang=fa&sid=1 شکوفه رنجبر 10031947532846002996 10031947532846002996 No دانشگاه آزاد اسلامی، اهواز مژگان خدادادی 10031947532846002997 10031947532846002997 Yes دانشگاه آزاد اسلامی، اهواز