Protected region of Margoon with area of 10000 hectar and mild weather with and different variety of plants and animals is one of the especialest protected places of Fars province; in other word, this region is very suitable for reviving the Ecosystem of Fars province. The objective of this research is to evaluate the ecological conditions of protected region of Margoon in order to developing the Ecotourism activities. The method of gathering descriptive-analytic data was done by library and documentary studies and in field data analysis. To achieve the objectives of this research the method of SWOT strategy was used base on the specifying the advantages, disadvantages, opportunities and threads and this method is weighted by the Analytic Hierarchy Process (AHP(.The present study which was conducted in 1391 Indicates that Margoon area had 16 domestic factors that weight rating of internal factors (6/55), the number of external factors was 15 factors and according to the assessment conducted weight score external factors (6/9). The number of external factors was 15 factors, and according to the assessment conducted, weight rating external factors (6/9) due to these conditions rating total factors are almost equal. According to the evaluation conducted, situation of Margoon Waterfall is in average condition. According to this research, the most important strategies to meet strengths and weaknesses, threats and opportunities have presented in the SWOT matrix. On this basis necessary investments in Section ecotourism and create ecotourism sites in surrounding area of Margoon waterfall and perform correct management and provide the necessary facilities and services, can be expanded the ecotourism in the area.