fa مرگ و میر دافنی ماگنا ( Daphnia magna) در مواجهه با نانو ذره اکسید کبالت) Co2O3) در محیط های آبی ، کاربردی اطمینان بخش از نشانگرهای زیستی در ارزیابی سمیت تخصصي Special پژوهشي Research <div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:115%"><span style="text-autospace:none"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:">با</span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:">توجه</span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:">به</span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:">تولید</span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:">روزافزون</span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:">نانو ذرات و کاربردهای</span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:">مفید</span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:">آنها</span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:">در</span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:">سیستم&shy;های</span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:">زیستی،</span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:">تاکنون</span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:">مطالعات</span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:">کمی</span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:">در زمینه</span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:">اثرات</span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:">جانبی</span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:">این</span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:">مواد بر موجودات زنده</span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:">صورت</span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:">گرفته</span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:">است</span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:BLotus">.</span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="background:white"><span style="line-height:115%"><span b="" mitra="" style="font-family:">خواص ویژه و منحصر به فرد نانو ذرات، خطرات احتمالی منحصر به فردی نیز به دنبال خواهد داشت</span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:">. از طرف دیگر نانو ذرات</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="background:white"><span style="line-height:115%"><span b="" mitra="" style="font-family:"> در شرایط مختلف ممکن است اثرات مختلفی داشته باشند</span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"> و با ورود به اکوسیستم&shy;های آبی به موجودات زنده آسیب برساند. از این رو در این مطالعه به منظور ارزیابی اثرات نانو ذرات در محیط&shy;های آبی، از نانو ذره &nbsp;اکسید کبالت (</span></span></span><span dir="LTR" new="" roman="" style="font-family:" times="">Co₂O₃</span><span b="" mitra="" style="font-family:"> ) </span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:">بر زئوپلانکتون سخت آب شیرین (</span></span></span><i><span dir="LTR" new="" roman="" style="font-family:" times="">Daphnia</span></i><i> </i><i><span dir="LTR" new="" roman="" style="font-family:" times="">magna</span></i><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:">)</span></span></span><span b="" lang="FA" mitra="" style="font-family:">، </span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:">در دو محیط آب مقطر و آب رودخانه کارون در شهر اهواز در سال 1397 &nbsp;استفاده شد. بر این اساس</span></span></span><i><span dir="LTR" new="" roman="" style="font-family:" times="">Daphnia</span></i><i> </i><i><span dir="LTR" new="" roman="" style="font-family:" times="">magna</span></i> <span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:">به مدت زمان &nbsp;96 ساعت در دمای 25-20 درجه سانتی&shy;گراد در معرض غلظت&shy;های 1، 5، 10، 20 ، 50 و</span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:">100 از نانو ذره قرار گرفت. پارامترهای مهم کیفی آب مانند اکسیژن محلول (</span></span></span><span dir="LTR" new="" roman="" style="font-family:" times="">DO</span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:">)، دما، اسیدیته (</span></span></span><span dir="LTR" new="" roman="" style="font-family:" times="">pH</span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:">)، طبق استاندارد در طول آزمایش کنترل شد.</span></span></span><span dir="LTR" new="" roman="" style="font-family:" times="">LC₅₀</span>&nbsp;<span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:">در زمان&shy;های 24، 48، 96 و 72 ساعت با استفاده از روش&shy;های </span></span></span><span dir="LTR" new="" roman="" style="font-family:" times="">Probit</span> <span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:">از نظر آماری بررسی شد. مقادیر</span></span></span><span dir="LTR" new="" roman="" style="font-family:" times="">LC₅₀ </span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:">&nbsp;96 ساعت برای&nbsp; نانو اکسید کبالت در آب مقطر و آب رودخانه کارون به&shy;ترتیب 04 /121 و 902/95 میلی&shy;گرم در لیتر بدست آمد. طبق نتایج این مطالعه، نرخ مرگ و میر </span></span></span><i><span dir="LTR" new="" roman="" style="font-family:" times="">&nbsp;D.magna</span></i><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:">در مواجهه با نانو ذره اکسید کبالت در نمونه&shy;های حاوی آب رودخانه بیشتر از نمونه&shy;های آب مقطر باشد که می&shy;تواند به علت وجود احتمالی سایر آلاینده&shy;ها و اثرات هم&shy;بیشی آنها در محیط&shy;های طبیعی باشد. همچنین مقادیر </span></span></span><span dir="LTR" new="" roman="" style="font-family:" times="">SAFE</span> <span b="" lang="FA" mitra="" style="font-family:">(</span><span dir="LTR" new="" roman="" style="font-family:" times="">Safety</span> <span dir="LTR" new="" roman="" style="font-family:" times="">Factor</span><span b="" lang="FA" mitra="" style="font-family:">)</span> <span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:">و </span></span></span><span dir="LTR" new="" roman="" style="font-family:" times="">SAR</span><span style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"> (</span></span></span><span dir="LTR" new="" roman="" style="font-family:" times="">Application</span><i> </i><span dir="LTR" new="" roman="" style="font-family:" times="">Rate</span> <span dir="LTR" new="" roman="" style="font-family:" times="">Safe</span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:">) برای این نانو ذره در هر دو محیط توصیه شد.</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""></span></span></span></span></span></span></span></span></span></div> <div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:115%"><span style="text-autospace:none"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><span new="" roman="" style="font-family:" times="">Due to the increasing production of nanoparticles and their useful applications in biological systems, so far few studies have been conducted on the side effects of these substances on living organisms. The special and unique properties of nanoparticles will also lead to unique potential hazards. On the other hand, nanoparticles may have different effects under different conditions and damage living organisms by entering aquatic ecosystems. Therefore, in this study, in order to evaluate the effects of nanoparticles in aqueous media, Co₂O₃ nanoparticles on hard freshwater zooplankton, <i>Daphnia magna</i>, were used in both distilled water and Karun River water in Ahvaz in 2018. Accordingly, Daphnia magna was exposed to concentrations of 1, 5, 10, 20, 50 and 100 nanoparticles for 96 hours at a temperature of 20-25 &deg; C. Important water quality parameters such as dissolved oxygen (DO), temperature, acidity (pH) were controlled according to the standard during the experiment. LC₅₀ was statistically analyzed at 24, 48, 96.72 hours using Probit methods. LC₅₀ values ​​of 96 hours for cobalt nano oxide in distilled water and Karunriver water were 121.04 and 95.902 mg /l, respectively. According to the results of this study, the mortality rate of <i>D.magna</i> in the presence of cobalt oxide nanoparticles in samples containing river water is higher than samples of distilled water, which may be due to the possible presence of other pollutants and their interaction effects in natural environments. . SAFE (</span><span new="" roman="" style="font-family:" times="">Safety Factor</span><span new="" roman="" style="font-family:" times="">) and SAR (</span><span new="" roman="" style="font-family:" times="">Safe Application<i> </i>Rate<i>)</i></span><span new="" roman="" style="font-family:" times="">) values ​​for this nanoparticle were also recommended in both environments.</span></span></span></span></span></span></div> سمیت حاد, نشانگر زیستی, Daphnia magna, رودخانه کارون, نانو اکسید کبالت Acute toxicity, Bioassay, Daphnia magna, Karun River, Cobalt Nano Oxide. 25 34 http://jweb.ahvaz.iau.ir/browse.php?a_code=A-10-699-2&slc_lang=fa&sid=1 لیلا فارسی 10031947532846006540 10031947532846006540 Yes مژگان خدادادی 10031947532846006541 10031947532846006541 No سیما سبزعلیپور 10031947532846006542 10031947532846006542 No نعمت اله جعفرزاده خقیقی فرد 10031947532846006543 10031947532846006543 No فرید جمالی شبنی 10031947532846006544 10031947532846006544 No