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Molecular Identification of Bothriocephalus acheilognathi (Family Bothriocephalidae) Isolated from Kazerun Shahpour River Fish using PCR Method
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Alireza Golchin Manshadi   , Mehdi Emadi , Maryamm Shahrivar |
| Islamic Azad University |
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Abstract: (27 Views) |
In order to identify fish parasitic cestodes from the Shahpour River and Seyed Hossein Spring in 2023, a total of 38 fish from four species Albumus mossulensis, Cyprinion macrostomum, Capoeta barroisi persica, and Garra rufa were caught and kept alive, then transferred to the Parasitology Laboratory of the Faculty of Veterinary Medicine at Islamic Azad University, Kazeroun Branch. The samples were examined in the parasitology laboratory of the Faculty of Veterinary Medicine at Kazerun. After anesthetizing the fish, biometric measurements were conducted for species identification, and their abdominal cavities and viscera were examined for the presence of cestodes. The results showed that only the Bothriocephalus sp. was isolated and identified from the intestine of the female A. mossulensis. 40% of the A. mossulensis were infected with Bothriocephalus sp., while the other fish were not infected. Subsequently, the Bothriocephalus sp., was studied using molecular methods. PCR amplification and nucleotide sequencing analysis of the ITS-1, 5.8S, and ITS-2 genes revealed an 88% similarity between the tested sample and the standard B. acheilognathi specimen with the accession number KP099579.1.
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Article number: 2 |
| Keywords: Cestode, Fish, Shahpour River, Kazerun, PCR |
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Full-Text [PDF 516 kb]
(16 Downloads)
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Type of Study: Research |
Subject:
Special
Received: 2025/05/2 | Accepted: 2025/06/28 | Published: 2025/06/29
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