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Effects of anesthesia withMS222, Eugenoland 2-Phenoxyethanol on some immune parameters of Cyprinuscarpio
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Mojtaba Alishahi   , Behnaz Cheshmeh , Rahim Peyghan , مسعود Ghorbanpour , Takavar Mohamadian |
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Abstract: (7365 Views) |
| Three anesthetic agents MS222, Eugenol and 2-Phenoxyethanol are the most drugs used in fish anesthesia but their effects on physiological and immunological parameters less investigated. In presentstudy thatperformed in 2012 in ShahidChamran University, 400 common carp weghining around 100 g were collected. The effective concentration of each anesthetics were evaluated first. Then the effects of anesthesia with MS222, Clove oil (Eugenia caryophyllata) and 2-Phenoxyethanol on some immune parameters of Cyprinuscarpio were investigated. Firstly, the effective doses of each anesthetic were calculated in Cyprinuscarpio. Fish were anaesthetized with the effective dose of each anesthetic and blood samples were taken at 0, 1, 12, 24 and 72 hours after anesthesia. Immunological parameters including: serum Lysozyme and antibacterial activity, complement activity, Nitrobluetetrazolium activity (NBT) as well as total serum immunoglobulin and protein were compared among the groups. Results showed that proper anesthetic dose of MS222, Clove oil and Phenoxyethanol in Cyprinuscarpiowere 100, 125 and 400 mg l-1 respectively. Serum lysozyme activity of fish anaesthetized with three anesthetics in different sampling periods showed no significant difference (P>0.05). Although serum bactericidal activity of fish anaesthetized with Phenoxinyethanol at 24 and 72 hours after anesthesia were significantly lower than control groups, bactericidal activity of other treatments showed no significant difference (P>0.05). Other immunological parameters including NBT and complement activity as well as total immunoglobulin and protein didn’t affected by anesthesia with these three Anesthetics(P>0.05). Results showedit couldconclude that although anesthesia with MS222 and Clove oil were no adverse effects on immunological parameters of Cyprinuscarpio, Phenoxyethanole cause light immunesuppression in Cyprinuscarpio. So Phenoxyethanole isn’t recommendable for anesthesia in immunological research in Cyprinuscarpio. |
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| Keywords: Common carp, Anesthesia, MS222, Clove oil, Phenoxyethanol, Immunological parameters, Cyprinuscarpio. |
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Full-Text [PDF 31 kb]
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Type of Study: Research |
Subject:
Special
Received: 2015/01/6 | Accepted: 2015/01/6 | Published: 2015/01/6
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