Euglenoids Algae are monocellularworldwideorganismsthat are usually found in freshwater habitats. Hoor-al-Azim or Hoor-al-Hawizeh is one of the most important wildlife and aquatic ecosystem that is shared between Iran (south west) and Iraq. It is part of Mesoptamian wetland that the various changes have during the last two decades had significant effects on the both structure and function of this wetland. In this study, identification and seasonal changes of Euglenophyta in Hoor-al-Azim wetland from April 2012to March 2012 was investigated. The sample were taken monthly from four stations. Then samplesfixed by Formalin 4%. Samples were identified by invert microscope. The frequency was also calculated. In this study a total of 18species belonging to four genera, Euglena, Phacus, Trachelomonas and Lepocincliswasidentified.The genus Euglena with 9 species had the highest number of species, as well as The winter season has the highest average density (1666cells per liter) and spring with the lowest average density (333cells per liter), respectively. Among all identified species, Lepocinclistexta with a total frequency of 42% and Euglena sp with a total frequency of 9% had the highest density than other species.