Journal of Wetland Ecobiology
Volume 17, Issue 2 (Summer 2025)
2025, 17(2): 12-24 |
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Avakh Keysami M, Rezaeian A, Moghateli M. Isolation and evaluation of Vibrio bacteria from some western white shrimp (Litopenaeus vannamei) farms in Delvar site, Bushehr. Wetland Ecobiology 2025; 17 (2) : 2
URL: http://jweb.ahvaz.iau.ir/article-1-1104-en.html
URL: http://jweb.ahvaz.iau.ir/article-1-1104-en.html
Agricultural Research, Education and Extension Organization
Abstract: (454 Views)
Vibrio species cause significant losses and damages, ranging from 50% to 100%, in shrimp farming facilities. Following the observation of certain disease symptoms and mortalities in cultured shrimp in the Delvar region of Bushehr, an investigation was conducted to isolate and evaluate Vibrio bacterial agents in the Whiteleg shrimp (Litopenaeus vannamei) cultured in this area. Sampling was performed on 300 Whiteleg shrimp. After microbial culture on general and selective media (TCBS, TSA), Vibrio species were isolated and identified using specific biochemical tests. The vibriostatic test (O/ 0.129) was conducted to differentiate the Vibrio genus from Aeromonas and many other Gram-negative species. The antibiogram test was performed using the disk diffusion method with disks containing specified amounts of the antibiotics Penicillin, Ampicillin, Oxytetracycline, Chloramphenicol, and Cephalothin. The physiological characteristics of the isolated bacteria were determined by examining the effects of temperature, salinity, and pH on their growth.Out of the 179 Vibrio sp. bacteria isolated from the cultured Whiteleg shrimp, 56 isolates were Vibrio parahaemolyticus, 48 were V. harveyi, 31 were V. alginolyticus, 21 were V. anguillarum, and 23 were related to other Vibrio spp. The results of the antibiogram test for the isolated bacterial species against the five antibiotics showed that the isolated bacteria were sensitive to Chloramphenicol, with inhibition zone diameters of 21.2 ± 0.3 to 26.4 ± 0.1 mm, Oxytetracycline, with diameters of 11.95 ± 0.1 to 13.85 ± 0.2 mm, and Cephalothin, with diameters of 9.56 ± 0.3 to 12.4 ± 0.1 mm, while they were resistant to Ampicillin and Penicillin. Since the physiological characteristics of the islated bacteria were consistent with the physicochemical conditions of the sampled farms, these bacteria, which are part of the shrimp's natural flora, become opportunistic and secondary pathogens in the presence of stress and compromised immune systems. They play a significant role in reducing cultured shrimp production. Therefore, in addition to reducing stressors for disease prevention and control, the effective antibiotics identified in this study can be used as potential therapeutic options, following definitive diagnosis and under veterinary supervision.
Article number: 2
Type of Study: Research |
Subject:
Special
Received: 2025/09/22 | Accepted: 2025/12/15 | Published: 2025/12/15
Received: 2025/09/22 | Accepted: 2025/12/15 | Published: 2025/12/15
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