Journal of Wetland Ecobiology
Volume 15, Issue 1 (Spring 2023)
2023, 15(1): 74-81 |
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The investigation of EDTA on the growth, Chlorophyll and carotenoids of Nannochloropsis oculata. Wetland Ecobiology 2023; 15 (1) : 6
URL: http://jweb.ahvaz.iau.ir/article-1-1136-en.html
URL: http://jweb.ahvaz.iau.ir/article-1-1136-en.html
Abstract: (232 Views)
Nannochloropsis oculata is one of the most important microalgae used in aquaculture. The present study aims to investigate the effects of different concentrations of EDTA on the growth and pigments of N. oculata. This study was conducted over 21 days in an environment with a temperature of 22 ± 1 °C, light intensity of 2500 lux (6 hours of light and 8 hours of darkness) and 24-hour ventilation with three repetitions. The basic culture medium was TMRL with values of 0, 0.01, 0.04, 0.08 and 0.12 g/l of EDTA solution and 25 ppt salinity. The algae density, amount of chlorophyll a, b, and carotenoids were measured. The maximum Chlorophyll a was found in EDTA level of 0.12 g/l in the fifteenth day, which was significantly different from other treatments. The increase of EDTA, however, did not have a significant effect on the amount of Chlorophyll b and the maximum amount was found in 0.08 g/l treatment in the seventh day. The highest rate of carotenoids was seen in 0.12 g/l treatment in the thirteenth day. Also, the maximum cell density was recorded in 0.12 g/l treatment in the fifteenth day. The results of this study showed that the increase of EDTA causes a rise in the amount of pigments, cellular growth, and the period of logarithmic phase, which is probably due to an increase in the pH of the culture medium, the properties of chelators and better absorption of nitrates by the microalgae.
Article number: 6
Type of Study: Research |
Subject:
Special
Received: 2026/01/16 | Accepted: 2026/01/20 | Published: 2026/01/20
Received: 2026/01/16 | Accepted: 2026/01/20 | Published: 2026/01/20
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